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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
06/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHEN, Z.; HAGEN, D. E.; WANG, J.; ELSIK, C. G.; JI, T.; SIQUEIRA, L. G. B.; HANSEN, P. J.; RIVERA, R. M. |
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Afiliação: |
Zhiyuan Chen, University of Missouri, Columbia, MO, USA; Darren E. Hagen, University of Missouri, Columbia, MO, USA; Juanbin Wang, University of Missouri, Columbia, MO, USA; Christine G. Elsik, University of Missouri, Columbia, MO, USA; Tieming Ji, University of Missouri, Columbia, MO, USA; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; Peter J. Hansen, University of Florida, Gainesville, FL, USA; Rocio M. Rivera, University of Missouri, Columbia, MO. |
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Título: |
Global assessment of imprinted gene expression in the bovine conceptus by next generation sequencing. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Epigenetics, v. 11, n. 7, p. 501-516, 2016. |
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Idioma: |
Português |
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Conteúdo: |
Abstract Genomic imprinting is an epigenetic mechanism that leads to parental-allele-specific gene expression. Approximately 150 imprinted genes have been identified in humans and mice but less than 30 have been described as imprinted in cattle. For the purpose of de novo identification of imprinted genes in bovine, we determined global monoallelic gene expression in brain, skeletal muscle, liver, kidney and placenta of day ∼105 Bos taurus indicus × Bos taurus taurus F1 conceptuses using RNA sequencing. To accomplish this, we developed a bioinformatics pipeline to identify parent-specific single nucleotide polymorphism alleles after filtering adenosine to inosine (A-to-I) RNA editing sites. We identified 53 genes subject to monoallelic expression. Twenty three are genes known to be imprinted in the cow and an additional 7 have previously been characterized as imprinted in human and/or mouse that have not been reported as imprinted in cattle. Of the remaining 23 genes, we found that 10 are uncharacterized or unannotated transcripts located in known imprinted clusters, whereas the other 13 genes are distributed throughout the bovine genome and are not close to any known imprinted clusters. To exclude potential cis-eQTL effects on allele expression, we corroborated the parental specificity of monoallelic expression in day 86 Bos taurus taurus × Bos taurus taurus conceptuses and identified 8 novel bovine imprinted genes. Further, we identified 671 candidate A-to-I RNA editing sites and describe random X-inactivation in day 15 bovine extraembryonic membranes. Our results expand the imprinted gene list in bovine and demonstrate that monoallelic gene expression can be the result of cis-eQTL effects. MenosAbstract Genomic imprinting is an epigenetic mechanism that leads to parental-allele-specific gene expression. Approximately 150 imprinted genes have been identified in humans and mice but less than 30 have been described as imprinted in cattle. For the purpose of de novo identification of imprinted genes in bovine, we determined global monoallelic gene expression in brain, skeletal muscle, liver, kidney and placenta of day ∼105 Bos taurus indicus × Bos taurus taurus F1 conceptuses using RNA sequencing. To accomplish this, we developed a bioinformatics pipeline to identify parent-specific single nucleotide polymorphism alleles after filtering adenosine to inosine (A-to-I) RNA editing sites. We identified 53 genes subject to monoallelic expression. Twenty three are genes known to be imprinted in the cow and an additional 7 have previously been characterized as imprinted in human and/or mouse that have not been reported as imprinted in cattle. Of the remaining 23 genes, we found that 10 are uncharacterized or unannotated transcripts located in known imprinted clusters, whereas the other 13 genes are distributed throughout the bovine genome and are not close to any known imprinted clusters. To exclude potential cis-eQTL effects on allele expression, we corroborated the parental specificity of monoallelic expression in day 86 Bos taurus taurus × Bos taurus taurus conceptuses and identified 8 novel bovine imprinted genes. Further, we identified 671 candidate A-to-I RNA edit... Mostrar Tudo |
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Palavras-Chave: |
A-to-I RNA editing; Allele-specific gene expression; Next generation sequencing; X chromosome inactivation. |
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Thesaurus Nal: |
genomic imprinting. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154835/1/Cnpgl-2016-Epigenetics-Global.pdf
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Marc: |
LEADER 02519naa a2200265 a 4500
001 2062831
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aCHEN, Z.
245 $aGlobal assessment of imprinted gene expression in the bovine conceptus by next generation sequencing.$h[electronic resource]
260 $c2016
520 $aAbstract Genomic imprinting is an epigenetic mechanism that leads to parental-allele-specific gene expression. Approximately 150 imprinted genes have been identified in humans and mice but less than 30 have been described as imprinted in cattle. For the purpose of de novo identification of imprinted genes in bovine, we determined global monoallelic gene expression in brain, skeletal muscle, liver, kidney and placenta of day ∼105 Bos taurus indicus × Bos taurus taurus F1 conceptuses using RNA sequencing. To accomplish this, we developed a bioinformatics pipeline to identify parent-specific single nucleotide polymorphism alleles after filtering adenosine to inosine (A-to-I) RNA editing sites. We identified 53 genes subject to monoallelic expression. Twenty three are genes known to be imprinted in the cow and an additional 7 have previously been characterized as imprinted in human and/or mouse that have not been reported as imprinted in cattle. Of the remaining 23 genes, we found that 10 are uncharacterized or unannotated transcripts located in known imprinted clusters, whereas the other 13 genes are distributed throughout the bovine genome and are not close to any known imprinted clusters. To exclude potential cis-eQTL effects on allele expression, we corroborated the parental specificity of monoallelic expression in day 86 Bos taurus taurus × Bos taurus taurus conceptuses and identified 8 novel bovine imprinted genes. Further, we identified 671 candidate A-to-I RNA editing sites and describe random X-inactivation in day 15 bovine extraembryonic membranes. Our results expand the imprinted gene list in bovine and demonstrate that monoallelic gene expression can be the result of cis-eQTL effects.
650 $agenomic imprinting
653 $aA-to-I RNA editing
653 $aAllele-specific gene expression
653 $aNext generation sequencing
653 $aX chromosome inactivation
700 1 $aHAGEN, D. E.
700 1 $aWANG, J.
700 1 $aELSIK, C. G.
700 1 $aJI, T.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aHANSEN, P. J.
700 1 $aRIVERA, R. M.
773 $tEpigenetics$gv. 11, n. 7, p. 501-516, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
30/07/1992 |
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Data da última atualização: |
04/10/2013 |
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Autoria: |
MATTOS, P. L. P. de. |
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Afiliação: |
PEDRO LUIZ PIRES DE MATTOS, CNPMF. |
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Título: |
Espaçamentos para a cultura da mandioca. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Cruz das Almas: EMBRAPA-CNPMF, 1991. |
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Páginas: |
2p. |
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Série: |
(EMBRAPA-CNPMF. Mandioca em foco, 37). |
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Idioma: |
Português |
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Conteúdo: |
Dentre as alternativas capazes de aumentar a produção de alimentos, fibras e matéria prima para a indústria de transformação, estão a expansão da fronteira agrícola e a elevação da produtividade. Dai urge a necessidade de melhoria do sistema de produção, com a introdução de práticas de cultivos corretas e de baixo custo. Em relação a cultura da mandioca, práticas como seleção do material de plantio, posição da maniva, plantio na época adequada e espaçamento, podem contribuir para um aumento na produtividade da ordem de 20 a 40% sem onerar os custos de produção. |
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Palavras-Chave: |
Cultivo; Uso. |
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Thesagro: |
Espaçamento; Mandioca; Manihot Esculenta; Pratica Cultural; Produção. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01146nam a2200217 a 4500
001 1636418
005 2013-10-04
008 1991 bl uuuu u0uu1 u #d
100 1 $aMATTOS, P. L. P. de
245 $aEspaçamentos para a cultura da mandioca.
260 $aCruz das Almas: EMBRAPA-CNPMF$c1991
300 $a2p.
490 $a(EMBRAPA-CNPMF. Mandioca em foco, 37).
520 $aDentre as alternativas capazes de aumentar a produção de alimentos, fibras e matéria prima para a indústria de transformação, estão a expansão da fronteira agrícola e a elevação da produtividade. Dai urge a necessidade de melhoria do sistema de produção, com a introdução de práticas de cultivos corretas e de baixo custo. Em relação a cultura da mandioca, práticas como seleção do material de plantio, posição da maniva, plantio na época adequada e espaçamento, podem contribuir para um aumento na produtividade da ordem de 20 a 40% sem onerar os custos de produção.
650 $aEspaçamento
650 $aMandioca
650 $aManihot Esculenta
650 $aPratica Cultural
650 $aProdução
653 $aCultivo
653 $aUso
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Embrapa Mandioca e Fruticultura (CNPMF) |
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